- Principal Investigator:
- Garry P. Nolan, Ph.D.
- Stanford University, Stanford, CA
- Garry Nolan
- cancer, cell signaling, immunology, leukemia, intracellular cytometry, mass cytometry, systems biology
About the lab
We analyze cell signaling directly by next-generation mass and traditional flow cytometry, focusing on following multiple phosphoproteins in complex populations of primary cells such as mouse cells and human clinical samples. Using mass cytometry, up to 34 simultaneous protein parameters can be measured in single cells including multiple kinases, phosphoproteins, cell cycle proteins, and other parameters, enabling resolution of cellular activation states.
We are using these techniques to study healthy biochemical signaling in the immune system and dysfunctional signaling in hematological malignancies including AML, ALL, JMML, MDS, follicular lymphoma and autoimmune diseases including rheumatoid arthritis and systemic lupus erythematosus. We are also using the technique for drug screening in primary cells to truly select for drugs with efficacies in certain cell subsets but not others.
About this resource
This resource was developed to facilitate dissemination of protocols and materials used in the lab as well as provide access to published articles linked to the underlying data and analysis via Cytobank Reports